Faculty Profile : DR. Rajesh Sonti
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Name: Dr. Rajesh Sonti
Assistant Professor
rajesh.sonti@niperhyd.ac.in
+91 40 23074750 Extn- 2025 +91-040-23074750 (Ext. No: 2013)
My Citations
Full Profile (pdf)
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ADDRESS, ROOM NO 
Department of Pharmaceutical Analysis
Room no: 51
NIPER Hyderabad
Balanagar, Hyderabad-500037, TS, INDIA
QUALIFICATION: (REVERSE CHRONOLOGICAL ORDER)
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03/2018 - Project management training based on IPMA standards, SPOL AG training center, Steinhausen, Switzerland
06/2015 - 06/2017 - DAS in European Centre of Pharmaceutical Medicine, University of Basel, Switzerland
06/2017 - GCP Training for Investigators and Study Teams, University of Basel, Switzerland
08/2008 - 10/2013 - PhD Chemical Biology, Prof. P. Balaram, Indian Institute of Science - Bangalore, India
06/2006 - 05/2008 - Masters in Chemistry, Sri Sathya Sai University - A.P, India
06/2003 - 05/2006 - B.Sc (Hons.) in Chemistry, Sri Sathya Sai University - A.P, India
EXPERIENCE 
02/2020 - Present : Working as Assistant Professor at NIPER, Hyderabad.
11/2018 - 02/2020 : Collaborative Scientist, Prof. R. Gruetter, CIBM - EPFL, Lausanne, Switzerland
11/2013 - 10/2018 : Postdoctoral fellow under Prof. S. Grzesiek, Biozentrum – Basel, Switzerland .
COURSES HANDLING(PHD/M.PHARM) 
M.S (Pharm):
PA-510: Topics in Pharmaceutical Analysis
PA-610: Pharmacopoeial Methods of Analysis
PA-620:Modern Instrumental Techniques for Evaluation of APIs and Drug Products.
PhD:
PA-730: CE and SFC in Pharmaceutical Analysis
PA-760: Mass spectrometry in Pharmaceutical Analysis
PHD STUDENTS/MASTERS STUDENTS
Masters: Guided: 17 ; Ongoing - 7 [Advisor]
Ph.D: Ongoing - 4 [Advisor]
SERB JRF: Ongoing - 1
AWARDS / HONOURS 
- Recipient of partial travel grant from University of Basel for 58th ENC held at Asilomar, CA, USA
- Selected for the EMBO 2013 travel grant of practical course on “Structure, dynamics and function of biomacromolecules"
- Recipient of ICMRBS student travel Grant for XXVth ICMRBS 2012 held at Lyon, France.
- Selected for the CSIR travel grant for XXVth ICMRBS 2012 held at Lyon, France.
- Selected for the CICS-INSA travel grant for XXVth ICMRBS 2012 held at Lyon, France.
- Recipient of ENC student travel award for ENC2011 held at Asilomar, CA, USA.
- Selected for the DST travel grant for ENC2011 held at Asilomar, CA, USA.
- Recipient of NMRS travel award for ENC2011 held at Asilomar, CA, USA.
- Qualified in the All India Gate 2007.
- Selected for summer internship at GE (SABIC –Plastics) analytical division in 2008.
RESEARCH AREAS/INTEREST
During his Ph.D. at IISC-Bangalore with Prof. P. Balaram, he has worked on designed and natural peptides that are hybrid beta-sheets, modified alpha-helices, and cyclic disulfide-bonded peptides. He has done the conformational analysis of several peptides and solved 23 structures of peptides by solution NMR. In Biozentrum, Basel-Switzerland, he worked with the protein, Abelson tyrosine kinase involved in Chronic Myeloid Leukemia in the group of Prof. Stephan Grzesiek. He tested the interaction of FDA-approved drugs, e.g., Gleevec (Novartis), Tasigna (Novartis), Sprycel (Bristol-Myers Squibb), Iclusig (ARIAD Pharmaceuticals), and Inlyta (Pfizer) that bind at the ATP binding site in a solution using NMR spectroscopy. These studies lead to the identification mechanism of Abl disassembled state upon binding of certain drugs from the assembled state. He characterized activities of several drug resistant mutants (‘gatekeeper,’ completely activated P242E/P249E), assessed their conformations, probed interdomain dynamics by 15N relaxation studies. At EPFL Laussane-Switzerland in the group of Prof. Rolf Gruetter, worked on the determination of lactate concentrations in the mice plasma and analyzed the fragmental labelling, 13C isotope enrichment by NMR. Furthermore, he worked on determining the concentrations of metabolites in mice cortex, hippocampus, and striatum regions using in vivo 9T MRS magnet. Further, European Centre of Pharmaceutical Medicine (ECPM-DAS) course at the University of Basel enhanced my knowledge and understanding of pharmaceutical medicine. With the best training and world-class experience received, he has started his career at NIPER-Hyderabad.
The scientific goal of my research is the application of analytical techniques such as Nuclear Magnetic Resonance (NMR), High resolution mass spectrometry (HRMS) for the elucidation of structure, and dynamics of peptides with a particular emphasis on understanding conformational diversity of novel conopeptides as a core area. The group is also interested in drug-drug interactions, metabolism of herbal drugs and drug stability studies. We collaborate with several scientists and provide analytical support in NIPER and outside. Further, we actively engage in consultancy projects for the structure elucidation of APIs and higher-order structure determination of biosimilars for pharmaceutical companies.
GRANTS/FELLOWSHIPS
- CCRUM project on “Structural characterization, pharmacokinetics, pharmacodynamic and herb-drug interactions of major constituents of compound Unani formulation for diabetes” awarded on 25 Apr 2024 - INR 28.17 Lakhs.
- DBT project “Therapeutic leads for pain from Conus peptides of Indian marine coast” awarded on 09 Nov 2023 - INR 89.58 Lakhs.
- DST SERB SRG awarded in December 2021 - INR 27 Lakhs.
- Co-recipient of Swiss Cancer League grant of three years in 2014, Biozentrum, University of Basel, Switzerland.
- Awarded CSIR Junior Research Fellowship Dec 2007 and Senior Research Fellowship in Aug 2010.
PUBLICATIONS / BOOK CHAPTERS
- S Sen, B Ganta, VN Rachel, SK Gogikar, V Singh, R Sonti, AG Dikundwar. Mapping Advantages and Challenges in Analytical Development for Fixed Dose Combination Products, a Review. Journal of Pharmaceutical Sciences, 2024, xxx, xxx.
- Rati Yadav, Rohit Bhawale, Vaibhavi Srivastava, Ekta Pardhi, Harshada Anil Bhalerao, Rajesh Sonti, Neelesh Kumar Mehra. Innovative Nanoparticulate Strategies in Colon Cancer Treatment: A Paradigm Shift. AAPS PharmSciTech, 2024, 25, 52.
- Mani Surya Kumar Palepu, Siva Nageswara Rao Gajula, Malleshwari K, Rajesh Sonti, Manoj P. Dandekar. SCFAs Supplementation Rescues Anxiety- and Depression-like Phenotypes Generated by Fecal Engraftment of Treatment-Resistant Depression Rats. ACS Chemical Neuroscience, 2024, 15, 1010.
- Paladini, Johannes; Maier, Annalena; Habazettl, Judith Maria; Hertel, Ines; Rajesh Sonti; Grzesiek, Stephan. The molecular basis of Abelson kinase regulation by its αI-helix. eLife, 2024.
- Dannarm, Srinivas Reddy; Harini, Dharipally; Reddy, Gangireddy Navitha; Ali, Nazish; Rajesh Sonti*. Study on the hydrolytic degradation behaviour of bictegravir by LC-PDA-Q/TOF-MS/MS NMR and in silico toxicity assessment. Journal of Pharmaceutical and Biomedical Analysis, 2024, 239, 115909.
- Ommi, Ojaswitha; Chilvery, Shrilekha; Dhopat, Priyanka Sudhir; Sharma, Anamika; Bhalerao, Harshada Anil; Dannaram, Srinivas Reddy; Nanduri, Srinivas; Rajesh Sonti; Godugu, Chandraiah; Yaddanapudi, Venkata Madhavi. Exploration of quinoxaline-benzimidazole hybrids as apoptosis-inducing agents and tubulin polymerisation inhibitors. Journal of Molecular Structure, 2023, 1292, 136184.
- Ojaswitha; Paoletti, Niccolò; Bonardi, Alessandro; Gratteri, Paola; Bhalerao, Harshada Anil; Sau, Shashikanta; Nanduri, Srinivas; Mohammed, Arifuddin; Kalia, Nitin Pal; Rajesh Sonti; Yaddanapudi, Venkata Madhavi; Exploration of 3‐aryl pyrazole‐tethered sulfamoyl carboxamides as carbonic anhydrase inhibitors. Archiv der Pharmazie, 2023, 356, 2300309.
- Dhurjad, Pooja; Gupta, Kajal; Sakla, Akash P; Shankaraiah, Nagula; Rajesh Sonti*. A validated high‐performance liquid chromatography method for the determination of brassinin, an indoleamine 2, 3‐dioxygenase inhibitor in rat plasma. Separation Science Plus, 2023, 6, 2300073.
- Shekh, Shamasoddin; Dhurjad, Pooja; Vijayasarathy, Marimuthu; Dolle, Ashwini; Dhannura, Shweta; Sahoo, Deepak Kumar; Rajesh Sonti; Gowd, Konkallu Hanumae. Oxidative Folding Catalysts of Conotoxins Derived from the Venom Duct Transcriptome of C. frigidus and C. amadis. Biochemistry, 2023, 62, 3061.
- Navitha Reddy, Gangireddy; Jogvanshi, Akanksha; Naikwadi, Sana; Rajesh Sonti*. Nirmatrelvir and ritonavir combination: an antiviral therapy for COVID-19. Expert Review of Anti-infective Therapy, 2023, 21, 943.
- Yakkala, Prasanna Anjaneyulu; Khan, Imran A; Dannarm, Srinivas Reddy; Aboti, Jyoti; Rajesh Sonti; Shafi, Syed; Kamal, Ahmed. Multicomponent Domino Reaction for Concise Access to 2-Amino-Substituted 1, 3, 4 Oxadiazoles via Smiles Rearrangement. The Journal of Organic Chemistry, 2023, 88, 12216.
- Rao Gajula, Siva Nageswara; Talari, Sasikala; Nathani, Tanaaz Navin; Munjal, Vijay; Rahman, Ziaur; Dandekar, Manoj P; Rajesh Sonti*. Effect of chronopharmacology and food on in vivo pharmacokinetic profile of mavacamten. Bioanalysis, 2023, 15, 695.
- Rao Gajula, Siva Nageswara; Godugu, Chandraiah; Rajesh Sonti*. Chronopharmacokinetics: a critical missing step in drug discovery and development. Bioanalysis, 2023.
- Rao Gajula, Siva Nageswara; Talari, Sasikala; Chilvery, Shrilekha; Godugu, Chandraiah; Rajesh Sonti*. A unique in vivo pharmacokinetic profile, in vitro metabolic stability and hepatic first-pass metabolism of garcinol, a promising novel anticancer phytoconstituent, by liquid chromatography–mass spectrometry. RPS Pharmacy and Pharmacology Reports, 2023, 2, 2.
- Siva Nageswara Rao Gajula, Ankita Sahebrao Khairnar, Pallavi Jock, Nikita Kumari, Kendre Pratima, Vijay Munjal, Pavan Kalan, Rajesh Sonti*. LC-MS/MS: A sensitive and selective analytical technique to detect COVID-19 protein biomarkers in the early disease stage. Expert Review of Proteomics, 2023, 1.
- Pooja Dhurjad, Pooja Jaiswal, Kajal Gupta, Parita Wanjari, Rajesh Sonti*. Mass spectrometry: A key tool in anti‐doping. Separation Science Plus, 2023, 6.
- Priyanka N Makhal, Srinivas Reddy Dannarm, Arbaz Sujat Shaikh, Rezwan Ahmed, Shrilekha Chilvery, Lahu N Dayare, Rajesh Sonti, Chandraiah Godugu, Venkata Rao Kaki. Exo-trig selenocyclization of secondary allylic carboxamides using Woollins’ reagent: en route to 2, 5-disubstituted selenazolines. Chemical Communications, 2023, 59, 3767.
- Srilakshmi Satti, Mani Surya Kumar Palepu, Aditya A Singh, Yash Jaiswal, Surya Prakash Dash, Siva Nageswara Rao Gajula, Sowmya Chaganti, Gananadhamu Samanthula, Rajesh Sonti, Manoj P Dandekar. Anxiolytic-and antidepressant-like effects of Bacillus coagulans Unique IS-2 mediate via reshaping of microbiome gut-brain axis in rats. Neurochemistry International, 2023.
- Bhoopendra Singh Kushwah, Hara Prasad Padhy, Rahul Khemchandani, Vijaya Madhyanapu Golla, Vinay Kumar Kanchupalli, Rajesh Sonti*, Gananadhamu Samanthula. Structural characterization of novel hydrolytic and oxidative degradation products of acalabrutinib by LC-Q-TOF-MS, H/D exchange and NMR. Journal of Pharmaceutical and Biomedical Analysis, 2022, 221, 115077.
- SNR Gajula, TN Nathani, RM Patil, S Talari, R Sonti*. Aldehyde oxidase mediated drug metabolism: an underpredicted obstacle in drug discovery and development. Drug Metabolism Reviews, 2022, 54, 427.
- P Dhurjad, CS Dhalaram, N Ali, N Kumari, R Sonti*. Metal-organic frameworks in chiral separation of pharmaceuticals. Chirality, 2022, 34, 1419-1436.
- Santosh K Sahoo, Siva NR Gajula, Mohammad N Ahmad, Grace Kaul, Srinivas Nanduri, Rajesh Sonti, Arunava Dasgupta, Sidharth Chopra, Venkata M Yaddanapudi. Bioevaluation of quinoline‐4‐carbonyl derivatives of piperazinyl‐benzothiazinones as promising antimycobacterial agents. Archiv der Pharmazie, 2022, 11, 2200168.
- SNR Gajula, SA Vora, AG Dikundwar, R Sonti*. In Vitro Drug Metabolism Studies Using Human Liver Microsomes. IntechOpen, 2022.
- Pratibha Anchi, Shrilekha Chilvery, Sayali Tekalkar, Siva Nageswara Rao Gajula, Rajesh Sonti*, Chandraiah Godugu. Nimbolide loaded sustained release microparticles as single-dose formulations for effective management of arthritis. Journal of Drug Delivery Science and Technology, 2022, 75, 103638.
- S Moi, S Shekh, KKA Reddy, P Dhurjad, R Sonti, KH Gowd. Peptide Cysteine Thiols Act as Photostabilizer of Avobenzone through Stabilizing the Transition State of Keto‐Enol Tautomerization. Photochemistry and Photobiology, 2022,
- SN Rao Gajula, NT Navin, S Talari, C Shende, R Sonti*. Green bioanalysis: an innovative and eco-friendly approach for analyzing drugs in biological matrices. Bioanalysis, 2022, 12, 881.
- Manoj P Dandekar, Mani Surya Kumar Palepu, Srilakshmi Satti, Yash Jaiswal, Aditya A Singh, Surya Prakash Dash, Siva Nageswara Rao Gajula, Rajesh Sonti. Multi-strain Probiotic Formulation Reverses Maternal Separation and Chronic Unpredictable Mild Stress-Generated Anxiety-and Depression-like Phenotypes by Modulating Gut Microbiome–Brain Activity in Rats. ACS Chemical Neuroscience, 2022, 13, 1948.
- Santosh K. S.; Sarvan, M..; Siva, N. R. G.; Mohammad. N. A.; Grace. K..; Srinivas, N.; Sonti. R; Arunava, D.; Sidharth, C.; Venkata M. Y., Identification of nitrofuranylchalcone tethered benzoxazole-2-amines as potent inhibitors of drug resistant Mycobacterium tuberculosis demonstrating bactericidal efficacy, Bioorganic & Medicinal Chemistry, 2022, 64, 116777.
- Grzesiek, S.; Paladini, J.; Habazettl, J.; Sonti. R, Imatinib disassembles the regulatory core of Abelson kinase by binding to its ATP site and not by binding to its myristoyl pocket, Magnetic Resonance, 2022, 3, 1.
- Gaurav. P.; Shaik, M. G.; Swayamsiddha, K.; Sai, M. C.; Dannarm, S. R.; Jitendra, G.; Sonti. R.; Srinivas. N, SmI2-mediated C-alkylation of Ketones with Alcohols in Microwave conditions: A Novel Route to Alkylated Ketones Chemistry - An Asian Journal, 2022, 17, 8.
- Makhal, P. N..; Dannarm, S. R..; Shaikh, A. S.; Sonti. R.; Kaki, V. R. TBHP‐Mediated Selenocyclization of N‐Allylbenzamides/Benzthioamides via In‐Situ Generation of “PhSeOH” Species; ChemistrySelect, 2022, 7, 14.
- Gaurav, P.; Shaik, M. G.; Swanand, V. J.; Preeti, R.; Swayamsiddha, K.; Mahesh, P. S.; Dannarm, S. R.; Sonti. R.; Srinivas. N, Cu(I)-Catalyzed Microwave-Assisted Multicomponent Reaction Towards Synthesis of Diverse Fluorescent Quinazolino[4,3-b ]quinazolin-8-ones and Their Photophysical Study; ChemistrySelect, 2022, 7, 14.
- Dhurjad, P.; Dhavaliker, C.; Gupta, K.; R. Sonti, Exploring Drug Metabolism by the Gut Microbiota: Modes of Metabolism and Experimental Approaches. Drug Metab Dispos. 2022, 50, 224.
- Darshana, Bora.; Reddy, Srinivas.; Stephy Elsa, John.; Sravani, Sana.; R. Sonti; N. Shankaraiah, Regioselective ortho-sulphonamidation of β-carbolines: Exploration of Intrinsic directing property of β-carbolines and their photophysical studies. Asian J. Org. Chem. 2021, 10, 3384.
- Gangireddy, Navitha Reddy.; Laltanpuii.; R. Sonti, Review on in vivo profiling of drug metabolites with LC-MS/MS in the past decade. Bioanalysis. 2021, 22, 1697.
- Rao, G. S. N.; Megha, S. P.; Samanthula, G.; R. Sonti, Cytochrome P450 enzymes: a review on drug metabolizing enzyme inhibition studies in drug discovery and development. Bioanlaysis. 2021, 13, 17.
- Rao, G. S. N.; Nimisha, N.; R. Sonti, Drug Metabolic Stability in Early Drug Discovery to Develop Potential Lead Compounds. Drug Metab Rev. 2021, 53, 49.
- Cherix A, Sonti R, Lanz B, Lei H. In Vivo Metabolism of [1,6- 13C2]Glucose Reveals Distinct Neuroenergetic Functionality between Mouse Hippocampus and Hypothalamus
Metabolites. 2021; 11, 50.
- Rao Gajula SN, Reddy GN, Reddy DS, Sonti R. Pharmacokinetic drug-drug interactions: an insight into recent US FDA-approved drugs for prostate cancer. Bioanlaysis. 2020;12, 1647.
- Sonti R, Ines HH, Lamontanara AJ, Hantschel O, and Grzesiek S. “ATP site ligands determine the assembly state of the Abelson kinase regulatory core via the activation loop conformation”. J. Am. Chem. Soc. 2018, 140, 1863.
- Yarava JR, Sonti R, Kantharaju K, Raghothama S and Ramanathan KV. “Solid-state NMR at natural isotopic abundance for the determination of conformational polymorphism - the case of designed β-turn peptides containing di-prolines Amino Acids”. Chem Commun. 2017, 53, 1317.
- Chandrappa S, Madhusudana Reddy MB, Sonti R, Basuroy K, Raghothama S, and Balaram P. “Directing peptide conformation with centrally positioned pre-organized dipeptide segments: studies of a 12-residue helix and β-hairpin”. Amino Acids. 2015, 47, 291.
- Sonti R, Rao KN, Chidanand S, Gowd KH, Raghothama S, and Balaram P. “Conformational analysis of a novel 20 membered cyclic peptide disulfide from Conus virgo with a WPW segment: Evidence for an aromatic-proline sandwich” Chem. Eur. J. 2014, 20, 5075.
- Sonti R, Dinesh B, Basuroy K, Ragothama S, Shamala N, and Balaram P. “C12 helices in long hybrid (αγ)n peptides composed entirely of unconstrained residues with proteinogenic sidechains” Org. Lett. 2014, 16, 1656.
- Sonti R, Gowd KH, Rao KN, Raghothama S, Rodriguez A, Perez JJ, and Balaram P. “Conformational diversity in contryphans from conus venom. Cis/trans isomerisation and aromatic/proline interactions in the 23 membered ring of a 7-residue peptide disulfide loop.” Chem. Eur. J. 2013, 45, 15175.
- Sonti R, Gopi HN, Muddegowda U, Raghothama S, and Balaram P. “A designed three stranded β-sheet in a α/β hybrid peptide” Chem. Eur. J. 2013, 19 5955.
- Sonti R, Rai R, Raghothama S, and Balaram P. “NMR analysis of cross strand aromatic interactions in an 8 residue hairpin and a 14 residue three stranded β-sheet peptide” J Phys Chem B. 2012, 116, 14207.
- Chandrappa S, Aravinda S, Raghothama S, Sonti R, Rai R, Harini VV, Shamala N and Balaram P. “Helix and hairpin nucleation in short peptides using centrally positioned conformationally constrained dipeptide segments” Org. Biomol. Chem. 2012, 10, 2815.
- Ganesh NV, Ragothama S, Sonti R, and Jayaraman N. “Ring expansion of oxyglycals. Synthesis and conformational analysis of septanoside-containing trisaccharides” J. Org. Chem. 2010, 75, 215.
PATENTS
INVITED TALKS 
- Gave a keynote lecture on “Importance of NMR for characterizing degradation impurities” for a special one-day symposium on the Current trends in NMR spectroscopy held at CSIR–IICTon 22 July 2024.
- Gave a talk on “peptides and impurity identification by NMR” at Revealing Molecular Mysteries through NMR Spectroscopy organized in partnership with CSIR-IICT Hyderabad and Bruker BioSpin on 20 June 2024.
- Gave a talk on “Importance of NMR for characterizing degradation impurities” for Mestrelab Research on 9 Feb 2024 in Hyderabad.
- Gave a talk on “Study on the hydrolytic degradation behavior of bictegravir by LC-PDA-Q/TOF-MS/MS, NMR and in silico toxicity assessment’ in CBMR Lucknow on 4 Feb 2024.
- Gave a talk on “Conformational analysis of novel conopeptides and recent insights of Abelson tyrosine kinase drug binding mechanism by NMR” in IISER Berhampur on 26 Feb 2023.
- Gave a webinar on Advanced tools to understand in vitro science and translate it close to in vivo
- NMR spectroscopy: An analytical technique for chemists and biologists, SRM University, Sikkim, 7 June 2021.
- Conformational analysis of cyclic peptides by NMR spectroscopy, Seethalakshmi Ramaswamy College, 27 February 2021.
- NMR spectroscopy for Genotoxic impurity profiling, JSS college of Pharmacy, Ooty, 10-12 November 2020.
- NMR spectroscopy, Bangalore University, 19-20 August 2020.
- Basics to Advanced Topics in One Dimensional NMR Spectroscopy, REVA university, Bangalore, 23 May 2020.
- Gave a talk on “Conformational analysis of peptide toxins and protein-drug interactions in solution by NMR spectroscopy” in December 2019 at NIPER Ahmedabad.
- Gave a talk on “NMR analysis of peptides and protein drug interactions” in March 2018 at CIBM, EPFL Lausanne
- Gave a talk on “NMR analysis of synthetic and natural peptides” in July 2013 at Biozentrum, Basel
- Gave a talk on “Conformational diversity of contryphans by solution NMR” in NMRS 2012, Bangalore
SPONSORED PROJECTS 
Structure elucidation of related 2- unknown impurities, Granules India Limited, Rs 3,87,248.
Feasibility studies of peptide for Orbicular pharmaceuticals Ltd, Rs 1,51,984.
QNMR for Orbicular pharmaceuticals Ltd, Rs 49,118.
LABORATORY FACILITIES DEVELOPED 
- NMR experiments for the structure elucidation of pharmaceutical compounds and bioactive peptides.
- Structural elucidation of alkaloids and terpenoids by UV, IR, Mass and NMR techniques
ADDITIONAL RESPONSIBILITIES 
- NMR facility in charge
- Chair, International relations, Consulting, Alumni affairs Committee
- Co-Chair, National Centre for Research and Development in Bulk Drug (NCRDBD)
- Chair, International relations, Consulting, Alumni affairs Committee
- Chief Warden, Boy's Hostel
- Member, Industry interaction and international collaboration cell
- Member, Board of Studies and Research
- Member, Stores and Purchase (Capital)
ANY OTHER INFORMATION